Alcaligenes denitrificans n.sp.

Metabolism of Alcaligenes denitrificans in biofilm vs planktonic cells.

AIMS To compare the effect of phosphorous concentration (200 mg P l(-1) and 20 mg P l(-1)) on the denitrifying efficiency of Alcaligenes denitrificans when in the form of planktonic cells or in the form of a biofilm, and to select the most adequate C/N ratio. METHODS AND RESULTS Two types of assays were carried out: with planktonic cells and with cells in biofilm form. Anoxic bottles with the...

Production and Purification of d-Aminoacylase from Alcaligenes denitrificans and Taxonomic Study of the Strain.

A d-aminoacylase-producing microorganism, strain DA181, isolated from soil was identified as Alcaligenes denitrificans subsp. denitrificans. This strain produced about 29,300 units (micromoles of product formed per hour) of d-aminoacylase and 2,300 units of l-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1% N-acetyl-dl-leucine as the carbon source. The d-ami...

In vitro susceptibility of Alcaligenes denitrificans subsp. xylosoxidans to 24 antimicrobial agents.

The in vitro susceptibilities of 37 clinical isolates of Alcaligenes denitrificans subsp. xylosoxidans to 24 antimicrobial agents were determined. Imipenem was the only drug with consistent activity (MIC for 90% of isolates, 2 micrograms/ml). Piperacillin, ticarcillin-clavulanic acid, ceftazidime, and co-trimoxazole were active against most strains. All the isolates were resistant to ampicillin...

-Amino Acid:Pyruvate Transaminase from Alcaligenes denitrificans Y2k-2: a New Catalyst for Kinetic Resolution of -Amino Acids and Amines

SOLUBLE FORMS OF AN ANTI-XYLELLA ANTIBODY AND STRAINS OF ALCALIGENES XYLOSOXIDANS DENITRIFICANS CAPABLE OF SECRETING THEM Project Leader:

Several methods were used to create soluble forms of a single chain antibody (scFv S1) that binds to the surface of the grape strains of Xylella fastidiosa (Xf). S1 fused to a pelB leader and secreted from E. coli. These forms were not secreted correctly and could not bind Xf in an ELISA. Maltose binding protein fusions of S1 were soluble and could be used to detect Xf in an ELISA. We also succ...